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| Status |
Public on Nov 27, 2019 |
| Title |
Single-nucleus RNA sequencing reveals transcriptional changes of hippocampal neurons in APP23 mouse model of Alzheimer’s disease |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Alzheimer’s disease (AD) is a progressive neurodegenerative disorder that mostly strikes the elderly. However, the exact molecular and cellular pathogenesis of AD, especially the dynamic changes of neurons during disease progression, remains poorly understood. Here we used single-nucleus RNA sequencing (snRNA-seq) to access the transcriptional changes of hippocampal neurons in APP23 mouse model of AD. We performed snRNA-seq using a modified Smart-seq2 technique on 3,280 neuronal nuclei from the hippocampus of young and aged APP23 and control mice and identified four distinct subpopulations. Comparative transcriptional analysis showed multiple changes in different subtypes of hippocampal neurons of APP23 mice in comparison to control mice, as well as the transcriptional changes in these neurons during disease progression. Our findings revealed multiple neuronal subtype-specific transcriptional changes that may lead to targets for future studies of AD.
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| Overall design |
revealed multiple neuronal subtype-specific transcriptional changes in 6-month old WT(wild type) mouse and 24-month old APP23 mouse
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| |
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| Contributor(s) |
Zhong S, Wang M, Zhan Y |
| Citation(s) |
31928331 |
| |
| Submission date |
Nov 26, 2019 |
| Last update date |
Feb 26, 2020 |
| Contact name |
Yong Shen |
| E-mail(s) |
yongshen@ustc.edu.cn
|
| Organization name |
University of Science and Technology of China
|
| Street address |
University of Science and Technology of China, No.96, JinZhai Road Baohe District
|
| City |
Hefei |
| ZIP/Postal code |
230022 |
| Country |
China |
| |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA592008 |
| SRA |
SRP233388 |
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