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| Status |
Public on Jul 31, 2020 |
| Title |
Single cell transcriptomes of pancreatic pre-invasive lesions and cancer reveal acinar metaplastic cells’ heterogeneity |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this project we have used single cell RNA-seq to profile pancreatic cancer development in a mouse model, from pre-invasive stage to cancer, and in human PDAC sample. Using a reporter gene, we were able to dissect metaplastic acinar cell heterogeneity, profiled six different acinar metaplastic cell types and states, validated their localization to pre-invasive lesions and correlated findings with human PDAC. In addition, we detected transcription factors, Onecut2 and Foxq1, that strictly expressed in metaplastic cells.
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| Overall design |
Tamoxifen was injected into six-eight weeks old Ptf1a-CreER, LSL-Kras-G12D, LSL-tdTomato (PRT) mice, and the pancreas was collected for single cell purification from mice at six different time points post-tamoxifen injection (PTI)
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| Contributor(s) |
Schlesinger Y, Parnas O, Yosefov-Levi O |
| Citation(s) |
32908137, 33649045 |
| |
| Submission date |
Nov 26, 2019 |
| Last update date |
Feb 02, 2022 |
| Contact name |
Oren Parnas |
| E-mail(s) |
orenp@ekmd.huji.ac.il
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| Organization name |
Hebrew University
|
| Department |
Immunology
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| Lab |
Oren Parnas Lab
|
| Street address |
Faculty of Medicine, Ein-Kerem medical center
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| City |
Jerusalem |
| ZIP/Postal code |
91120 |
| Country |
Israel |
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| Platforms (3) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA604712 |
| SRA |
SRP247080 |
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