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| Status |
Public on Nov 26, 2019 |
| Title |
Diet-induced changes in tuft cell dynamics couple with type 2 immune response modulation and energy metabolism |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: To examine the temporal changes in intestinal tuft cell number and activity in response to intake of a high fat diet and investigate the relation to whole-body energy metabolism and the immune phenotype of the small intestine and epididymal white adipose tissue (eWAT).
Methods: C57BL/6J mice were fed either a low fat reference diet (RFD, 10 % kcal fat) or a high fat diet (HFD, 60% kcal fat) for 9 or 22 weeks, followed by characterization of whole-body metabolic parameters, transcription profiles of sorted intestinal tuft cells, and immune phenotypes of tissues.
Results: HFD feeding was associated with reductions in the overall number of small intestinal epithelial cells and tuft cells and reduced expression of the intestinal type 2 tuft cell markers Il25 and Tslp. Amongst >1,700 diet-regulated genes in tuft cells identified by RNA-seq, we observed an early association between body mass expansion and increased expression of Serpini1, which encodes the serine protease inhibitor neuroserpin. By contrast, tuft cell expression of genes encoding the GABA-receptors linked to reduced body mass gain. Although we observed a HFD-induced change in the eWAT inflammatory profile, the small intestinal lamina propria displayed a consistent non-inflammatory phenotype, and small intestinal tuft cell-derived transcripts were found to correlate with whole body metabolic features independently of intestinal immune cell involvement.
Conclusion: Our These findings point to a role for small intestinal tuft cells in systems-wide metabolic regulation.
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| Overall design |
We here aimed to characterize the functional aspects of small intestinal tuft cells in response to HFD feeding with a focus on their role in relaying immune-metabolic signals. Additionally, we explored how HFD-associated effects on small intestinal tuft cells coupled with small intestinal and eWAT immune profiles as well as whole-body metabolic parameters
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| Contributor(s) |
Arora P, Andersen D, Moll JM, Danneskiold-Samsøe NB, Xu L, Zhou B, Kladis G, Rausch P, Workman CT, Kristiansen K, Brix S |
| Citation(s) |
34122403 |
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| Submission date |
Nov 25, 2019 |
| Last update date |
Jun 15, 2021 |
| Contact name |
Pankaj Arora |
| E-mail(s) |
sbrix@dtu.dk, sbp@bio.dtu.dk, panar@dtu.dk, panar.arora@sund.ku.dk
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| Phone |
+45 45252784
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| Organization name |
Technical University of Denmark
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| Department |
Department of Biotechnology and Biomedicine
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| Street address |
Søltofts Plads - blg. 224 room 218, DK-2800 Kgs. Lyngby
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| City |
Lyngby |
| ZIP/Postal code |
2800 |
| Country |
Denmark |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (45)
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| Relations |
| BioProject |
PRJNA591639 |
| SRA |
SRP233226 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE141002_FPKM.gene.matrix_LTF_Arora_et_al.2019.txt.gz |
1.6 Mb |
(ftp)(http) |
TXT |
| GSE141002_FPKM.gene.matrix_STF_Arora_et_al.2019.txt.gz |
1.4 Mb |
(ftp)(http) |
TXT |
| GSE141002_ReadCount.gene.matrix_LTF_Arora_et_al.2019.txt.gz |
1.0 Mb |
(ftp)(http) |
TXT |
| GSE141002_ReadCount.gene.matrix_STF_Arora_et_al.2019.txt.gz |
939.6 Kb |
(ftp)(http) |
TXT |
| GSE141002_TPM.gene.matrix_LTF_Arora_et_al.2019.txt.gz |
1.6 Mb |
(ftp)(http) |
TXT |
| GSE141002_TPM.gene.matrix_STF_Arora_et_al.2019.txt.gz |
1.4 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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