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| Status |
Public on Mar 03, 2020 |
| Title |
A mammalian Wnt5a–Ror2–Vangl2 axis controls the cytoskeleton and confers cellular properties required for alveologenesis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Alveolar formation increases the surface area for gas-exchange and is key to the physiological function of the lung. Alveolar epithelial cells, myofibroblasts and endothelial cells undergo coordinated morphogenesis to generate epithelial folds (secondary septa) within the saccules to form alveoli. A mechanistic understanding of alveologenesis remains incomplete. We found that the planar cell polarity (PCP) pathway is required in both alveolar epithelial cells and myofibroblasts for alveologenesis. Our studies uncovered a Wnt5a–Ror2–Vangl2 cascade that endows cellular properties and thus novel mechanisms of alveologenesis. This includes PDGF secretion from alveolar type I and type II cells, cell shape changes of type I cells and migration of myofibroblasts. All these cellular properties are conferred by changes in the cytoskeleton and represent a new facet of PCP function. These results extend our current model of PCP signaling from polarizing a field of epithelial cells to conferring new properties at subcellular levels to regulate collective cell behavior.
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| Overall design |
Build the signal pathway axis of Ror2 - Vangl2 in lung alveolar development
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| |
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| Contributor(s) |
Zhang K, Yao E, Chuang P |
| Citation(s) |
32394892 |
| |
| Submission date |
Nov 21, 2019 |
| Last update date |
May 19, 2020 |
| Contact name |
Pao-Tien Chuang |
| Organization name |
University of California, San Francisco
|
| Street address |
555 Mission Bay Blvd. South
|
| City |
San Francisco |
| ZIP/Postal code |
CA 94158-3118 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA590893 |
| SRA |
SRP231147 |
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