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| Status |
Public on Feb 05, 2020 |
| Title |
Imp/IGF2BP levels modulate individual neural stem cell growth and division through myc mRNA stability |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The numerous neurons and glia that form the brain originate from tightly controlled growth and division of neural stem cells, regulated systemically by known extrinsic signals. However, the intrinsic mechanisms that control the characteristic proliferation rates of individual neural stem cells are unknown. Here, we show that the size and division rates of Drosophila neural stem cells (neuroblasts) are controlled by the highly conserved RNA binding protein Imp (IGF2BP), via one of its top binding targets in the brain, myc mRNA. We show that Imp stabilises myc mRNA leading to increased Myc protein levels, larger neuroblasts, and faster division rates. Declining Imp levels throughout development limit myc mRNA stability to restrain neuroblast growth and division, while heterogeneous Imp expression correlates with myc mRNA stability between individual neuroblasts in the brain. We propose that Imp-dependent regulation of myc mRNA stability fine-tunes individual neural stem cell proliferation rates.
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| Overall design |
RNA-seq and RIP-seq in Drosophila L3 larval brain.
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| |
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| Contributor(s) |
Samuels T, Järvelin AI, Davis I |
| Citation(s) |
31934860 |
| |
| Submission date |
Nov 19, 2019 |
| Last update date |
Feb 07, 2020 |
| Contact name |
Aino Inkeri Järvelin |
| Organization name |
University of Oxford
|
| Department |
Department of Biochemistry
|
| Street address |
South Parks Road
|
| City |
Oxford |
| ZIP/Postal code |
OX1 3QU |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL19528 |
Ion Torrent Proton (Drosophila melanogaster) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA591594 |
| SRA |
SRP233205 |
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