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| Status |
Public on Apr 02, 2020 |
| Title |
Next Generation Sequencing Facilitates Quantitative Analysis of PHB-knockdown and PHB-knockout Spermatocytes Transcriptomes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We analyzed the transcriptomic differences of cultured mouse spermatocytes (GC2 cells) stably transfected with PHB-targeting shRNA (called PHB KD GC2) from those with a control shRNA (called Ctrl GC2). Furthermore, we also analyzed the difference in the transcriptomes of spermatocytes between Phb conditional knock-out and control mice.
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| Overall design |
Examination of mRNAs in Ctrl GC2 cells, PHB KD GC2 cells and spermatocytes from Phb conditional knock-out and control mice. Three biological replicates of each sample were analyzed.
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| Contributor(s) |
Zhang L, Chen H |
| Citation(s) |
32232334 |
| |
| Submission date |
Nov 13, 2019 |
| Last update date |
Apr 02, 2020 |
| Contact name |
Lingfei Zhang |
| E-mail(s) |
lingfeizhang@sibcb.ac.cn
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| Organization name |
Shanghai Medical College Fudan University
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| Street address |
138 Yixueyuan Road
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| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
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| Platforms (1) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA589316 |
| SRA |
SRP229743 |
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