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Series GSE140151 Query DataSets for GSE140151
Status Public on Nov 09, 2019
Title Butyrate-induced LncRNA lncly6c promotes Ly6Chigh to Ly6Clow/neg macrophages through lncly6c /C/EBP/Nr4A-1 axis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Gut macrophages play a critical role in maintaining the homeostasis of gut immunes. The inflammatory macrophages generally are differentiated into resident macrophages to realize their function in gut tissues. But it is unclear how these inflammatory macrophages to differentiate resident macrophages. We here found that lycLy6c, a novel ultraconserved lncRNA, may promote the differentiation of gut inflammatory macrophage into gut resident macrophages. We demonstrate gut microbiota metabolites butyrate may upregulate the expression of lncly6c. Lncly6c not only binds with transcription factor C/EBPβ but also binds with multiple components of enzyme complexes, which promote the accumulation of H3K4me3 markers on the promoter region of NrA4-1. As a result, lncly6c causes the upregulation of Nr4A-1 to promote inflammatory macrophage to differentiate the resident macrophages. These results not only offer an evidence for gut microbiota to maintaining the gut homeostasis but also suggest a potential target for the therapy for colitis-associated diseases.
Overall design 10 × Genomics scRNA-seq was performed To further understand the effects of lncly6c on the differentiation of macrophages.
Contributor(s) Gao Y, qi H, Wei J, Yang Y, Liu X, Zhang Y, Yang R
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Submission date Nov 08, 2019
Last update date Nov 11, 2019
Contact name Ming Wei
Organization name Nankai University
Department Department of Immunology, Nankai University School of Medicine
Lab Rongcun Yang Lab
Street address 94 Weijin road
City Tianjin
ZIP/Postal code 300071
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (2)
GSM4155006 scRNA-Seq_WT /Colon
GSM4155007 scRNA-Seq_17KO /Colon
BioProject PRJNA588430
SRA SRP229357

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Supplementary file Size Download File type/resource
GSE140151_RAW.tar 57.2 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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