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Series GSE140111 Query DataSets for GSE140111
Status Public on Jan 13, 2020
Title B cell-intrinsic epigenetic modulation of antibody responses by dietary fiber-derived short-chain fatty acids
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary Short-chain fatty acids (SCFAs) butyrate and propionate are metabolites from dietary fibers fermentation by gut microbiota that can affect differentiation or functions of T cells, macrophages and dendritic cells. We show here that these SCFAs directly impact B cells to modulate in a dose-dependent fashion AID and Blimp1 expression, class-switch DNA recombination, somatic hypermutation and plasma cell differentiation, thereby impairing, through B cell-intrinsic activity, local (intestinal) and systemic T-dependent and T-independent antibody responses. In human and mouse B cells, butyrate and propionate upregulate select miRNAs that target Aicda and Prdm1 mRNA-3’UTRs through epigenetic inhibition of histone deacetylation of the respective miRNA host genes. Further, they modulate B cell Aicda and Prdm1 by acting as HDAC inhibitors, not as energy substrate or through GPR-engagement signaling. Finally, butyrate and propionate epigenetic impact on B cells extends to inhibition of autoantibody production and autoimmunity in lupus MRL/Faslpr/lpr and NZB/WF1 mice.
Overall design Analysis of the mRNA and micrRNA expression profiles in human B cells stimulated with CD154 plus IL-4 and IL-21 in the presence of nil or short-chain fatty acid butyrate
Contributor(s) Casali P, Zan H, Chen Y, Mohammad TA
Citation(s) 31896754
Submission date Nov 07, 2019
Last update date Jan 14, 2020
Contact name Yidong Chen
Phone 2105629163
Organization name UT Health Science Center at San Antonio
Department Population Health Sciences
Street address 8403 Floyd Curl Drive, MSC 7784
City San Antonio
State/province Texas
ZIP/Postal code 78229
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (4)
GSM4154562 set2IgGB1000_mRNA
GSM4154563 set2IgGNil_mRNA
GSM4154564 set2IgGB1000_smRNA
BioProject PRJNA588290
SRA SRP229120

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE140111_RAW.tar 190.0 Kb (http)(custom) TAR (of TXT)
GSE140111_allSample_miRs_ReadCount.txt.gz 3.1 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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