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| Status |
Public on Oct 26, 2020 |
| Title |
Chromatin-associated RNA of HSV-1 infected primary human fibroblasts including infection with vhs mutants |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Primary human foreskin fibroblasts (HFF) were infected with wild-type herpes simplex virus 1 (HSV-1) strain 17 or its vhs null mutant, or with BAC-derived viruses expressing either a wild-type or catalytically inactive vhs with the D195N mutation at a multiplicity of infection (MOI) of 10. Chromatin-associated RNA was prepared and subjected to RNA-seq.
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| Overall design |
HFF were infected with HSV-1 strain 17, its vhs-null mutant, BAC-derived strain 17 (WT-BAC), or BAC-derived strain 17 with the vhsD195N mutation (WT-BAC VhsD195N) for 8h (or mock). Chromatin-associated RNA was prepared combining the following two protocols (PMID: 19656867, RNA 2009; and PMID: 23449254, Nature Protocols 2013) as described in Hennig et al., PLoS Pathogens 2018 (https://doi.org/10.1371/journal.ppat.1006954). Two independent biological replicates were analysed.
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| Contributor(s) |
Friedel CC, Whisnant AW, Djakovic L, Rutkowski AJ, Friedl M, Kluge M, Sai S, Oliveira Vidal R, Hennig T, Sauer S, Erhard F, Dölken L |
| Citation(s) |
33148793 |
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| Submission date |
Nov 07, 2019 |
| Last update date |
Jan 25, 2021 |
| Contact name |
Caroline Friedel |
| Organization name |
Ludwig-Maximilians-Universität München
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| Department |
Institut für Informatik
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| Street address |
Amalienstr. 17
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| City |
München |
| ZIP/Postal code |
80333 |
| Country |
Germany |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA588169 |
| SRA |
SRP229076 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE140068_gene_counts.tsv.gz |
608.2 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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