|
Status |
Public on Nov 06, 2019 |
Title |
ATAC-seq profile of mouse palatal epithelium at E14.5 |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
Summary |
To profile nucleosome free regions of moust palatal epithelium at E14.5, we performed ATAC-seq for isolated palatal epithelium and mesenchyme cells.
|
|
|
Overall design |
We digested the mouse embryonic palate at E14.5 with 0.25% trypsin-EDTA (Life Technologies) at 4°C for 10 minutes, after which the reaction was stopped using trypsin Inhibitor (Life Technologies). Under a dissecting microscope, the epithelium was isolated for ATAC-seq by gently peeling using microforceps. The remaining tissue, comprised largely of mesenchymal cells, was also collected for control samples. For each biological replicates, 20000 cells from 3 different mouse embryos were used. Three biological replicates were sequenced using HiSeq X Ten.
|
|
|
Contributor(s) |
Liu H, Cornell RA |
Citation(s) |
32031521 |
|
Submission date |
Nov 05, 2019 |
Last update date |
Oct 07, 2020 |
Contact name |
Robert Cornell |
E-mail(s) |
robert-cornell@uiowa.edu
|
Organization name |
University of Iowa
|
Department |
Anatomy and Cell Biology
|
Street address |
1-400 D Bowen Science Bldg. 51 Newton Road
|
City |
Iowa City |
State/province |
Iowa |
ZIP/Postal code |
52242 |
Country |
USA |
|
|
Platforms (1) |
|
Samples (6)
|
|
Relations |
BioProject |
PRJNA587709 |
SRA |
SRP228592 |