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| Status |
Public on Nov 06, 2019 |
| Title |
The transcriptomic differences between Th1, Tr1, Treg, and Tneg cells in Leishmania donovani-infected mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This study was designed to determine the differences in gene transcripts between CD4+ T helper (Th)1, Tr1, Treg, and Tneg cells in L. donovani-infected mice.
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| Overall design |
Bulk RNA-sequencing was performed on CD4+ T helper (Th)1 (IFNγ+ IL-10-), Tr1 (IFNγ+ IL-10+), Treg (IFNγ- IL-10+), and Tneg (IFNγ- IL-10-) cells sorted from the spleen of n = 5 mice at day 14 of infection with L. donovani. Cells were stimulated with phorbol 12-myristate 13-acetate (PMA) and ionomycin prior to sorting by cytokine capture flow cytometry.
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| Contributor(s) |
Engwerda CR, Edwards CL, Ng SS |
| Citation(s) |
37917177 |
| |
| Submission date |
Nov 05, 2019 |
| Last update date |
Feb 09, 2024 |
| Contact name |
Susanna Ng |
| E-mail(s) |
susanna.ng@qimrberghofer.edu.au
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| Organization name |
QIMR Berghofer Medical Research Institute
|
| Department |
Immunology
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| Lab |
Immunology and Infection Lab
|
| Street address |
300 Herston Road
|
| City |
Herston |
| State/province |
QLD |
| ZIP/Postal code |
4006 |
| Country |
Australia |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA587650 |
| SRA |
SRP228581 |
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