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Series GSE139890 Query DataSets for GSE139890
Status Public on Nov 02, 2020
Title Zinc effect on mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary This study underlines the beneficial use of excess dietary zinc in preventing colitis and inflammatory events. Zn-induced gene expression profiles were analyzed at ileum and colon level. A total number of 101 profiles were found differentially expressed during Zn supplemented diet, with an unbalanced pattern between these two tissues. A largest number of genes were found up-regulated in colon (22 genes), unlike the ileum (6 genes). At the other side, the number of down-regulated genes was higher in ileum (63 genes) against the colon (15 genes). Very few common profiles were detected in these two tissues, 3 up- and 2 down-regulated genes respectively. Genes coding for metallothioneins (Mt1, Mt2) and for phosphatidylinositol-4-phosphate 5-kinase (Pip5k1a) were found commonly up-regulated in ileum and colon, with the highest FC values. Genes coding for the zinc transporter of solute carrier family 39 (Slc39a4) and for lymphotoxin B (Ltb) were found commonly down-regulated with among the lowest FC values. Despite the different patterns, functional clustering of expressed genes converged for substantially the same physiological activities in both ileum and colon: intestinal tissue protection and epithelium reestablishment, immune system regulation as well as digestive and metabolic targeted functions.
Overall design Three groups of mice were randomized to receive distinct doses of ZnCl2 solutions administered in the drinking water while a control group consisted in standard uncontaminated drinking water. After 15 days of Zn treatment, mice are sacrificed and sections from the median colon and terminal ileum were collected and processed for transcriptomic analyses.
Contributor(s) Lucau-Danila A, Foligné B, Daniel C
Citation Foligné B, George F, Staendaert A, Garat A, Poiret S, Peucelle V, Ferreira S, Sobry H, Muharram G, Lucau-Danila A, Daniel C. (2020) High-dose dietary supplementation with zinc prevents gut inflammation: investigation of the role of metallothioneins and beyond by transcriptomic and metagenomic studies. The FASEB Journal 00: 1–19. DOI: 10.1096/fj.202000562RR.
Submission date Nov 04, 2019
Last update date Nov 04, 2020
Contact name Anca Lucau-Danila
Organization name University of Lille
Department Biology
Lab Charles Viollette Institute
Street address Cité Scientifique SN2 303
City Villeneuve d'Ascq
ZIP/Postal code 59655
Country France
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (16)
GSM4148354 Ileum_001
GSM4148355 Ileum_002
GSM4148356 Ileum_003
BioProject PRJNA587484

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE139890_Processed_data.xlsx 26.5 Kb (ftp)(http) XLSX
GSE139890_RAW.tar 61.6 Mb (http)(custom) TAR (of TXT)
GSE139890_matrix_CM.txt.gz 482.4 Kb (ftp)(http) TXT
GSE139890_matrix_IL.txt.gz 457.6 Kb (ftp)(http) TXT
Processed data are available on Series record

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