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| Status |
Public on Feb 01, 2009 |
| Title |
Effects of labelling fetal MSC with MRI Particles: MGIO and Ferucarbotran |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Cell tracking is enabled by incubating ex vivo cells with commercially/clinically available MRI particulate label, such as ferucarbotran. However, the uptake by non-phagocytic cells, such as mesenchymal stem cell (MSC) is poor, and the detection by MRI is impeded. MGIO is a new label that is efficiently taken up by MSC. The proliferation and differentiation capacity of labelled cells are usually assessed to determine cytotoxicity. In this study, we compared the global gene expression profiles of mock-labelled, ferucarbotran-labelled and MGIO-labelled fetal MSC.
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| Overall design |
Fetal MSC were labelled at passage 5, washed with PBS to remove excess labels, detached by trypsin, pelleted by centrfugation and lysed for RNA extraction with the RNeasy mini kit (Qiagen) in triplicates.
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| Contributor(s) |
Lee E, Chan J, Wang S |
| Citation(s) |
19544438 |
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| Submission date |
Dec 15, 2008 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Eddy Shoo-Ming Lee |
| Organization name |
University of Queensland
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| Lab |
UQCCR
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| Street address |
Royal Brisbane and Women's Hospital
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| City |
Herston |
| State/province |
Queensland |
| ZIP/Postal code |
4029 |
| Country |
Australia |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA110229 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE13975_RAW.tar |
47.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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