|
| Status |
Public on Aug 14, 2020 |
| Title |
CRISPR/Cas9 mediated intersectional knockout of GSK3b in D2 receptor expressing mPFC neurons reveals contributions to emotional regulation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Comparison of ribosome associated RNA between mPFC D2 neurons that lack Gsk3β with Controls
|
| |
|
| Overall design |
Gsk3β was knockout in mPFC D2 neurons using CRISPR/Cas9, than ribosome associated RNA was isolated from Control and knockout cells using RiboTag immunoprecipitation and subjected to RNAseq
|
| |
|
| Contributor(s) |
Khlghatyan J, Beaulieu J |
| Citation(s) |
32584144 |
| |
| Submission date |
Oct 29, 2019 |
| Last update date |
Aug 14, 2020 |
| Contact name |
Martin Beaulieu |
| E-mail(s) |
martin.beaulieu@utoronto.ca
|
| Phone |
4189297741
|
| Organization name |
University of Toronto
|
| Department |
Pharmacology and Toxicology
|
| Lab |
Martin Beaulieu
|
| Street address |
1 king's college circle room 4336
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5S 1A8 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA580259 |
| SRA |
SRP227366 |
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