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Series GSE139559 Query DataSets for GSE139559
Status Public on Jan 10, 2020
Title Myeloid Dendritic Cells from People Living with HIV have Altered Gene Expression Not Restored by Anti-Retroviral Therapy
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Myeloid dendritic cells (mDC) were isolated from antiretroviral therapy (ARV)-treated and untreated people living with HIV (PLWH), and from HIV uninfected Individuals. The results indicate that mDC are altered in genes expression from PLWH. Some RNA transcriptional changes are not completely restored by ARV. This provides more data on myeloid cells, an understudied cell type, and alterations in PLWH.
 
Overall design Peripheral blood mononuclear cells were enriched and flow cytometry-sorted to obtain purified myeloid dendritic cells from each of the donor groups. mRNA was isolated from the myeloid dendritic cells for each donor group, which included ART-treated (n=3) and Treatment-naive (n=3) PLWH, and HIV uninfected (n=4) persons. All samples were sorted with at least 2 technical replicates and microarray analyses were performed.
 
Contributor(s) Murray SM, Douek DC, Sekaly RP
Citation(s) 32194550
Submission date Oct 29, 2019
Last update date Mar 30, 2020
Contact name Shannon Murray
E-mail(s) smurray1@uw.edu
Phone (954) 560-0798
Organization name Fred Hutchinson Cancer Research Center
Street address 1100 Fairview Ave N
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platforms (2)
GPL6883 Illumina HumanRef-8 v3.0 expression beadchip
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (34)
GSM4143695 mDC_HIVnegativeDonor 1_replicate1
GSM4143696 mDC_HIVnegativeDonor 1_replicate2
GSM4143697 mDC_HIVnegativeDonor 1_replicate3
Relations
BioProject PRJNA580248

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE139559_RAW.tar 30.1 Mb (http)(custom) TAR
GSE139559_non-normalized.txt.gz 3.2 Mb (ftp)(http) TXT
GSE139559_normalized.txt.gz 1.4 Mb (ftp)(http) TXT
Processed data are available on Series record
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