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| Status |
Public on Oct 25, 2019 |
| Title |
Investigating the Interleukin-22 regulated transcriptome in normal and APC-mutant small intestinal epithelial organoids |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: to investigate the effects of the cytokine Interluekin-22 (IL-22) on small intestinal epithelial cells, using organoids.
Methods: WT or Apc(Min/Min) organoids (day 3) were stimulated with IL-22 (2ng/ml) for 3 hours, and submitted for transcriptomic profiling.
Results: The main function of IL-22 in small intestinal epithelial cells is to activate an antimicrobial immune response and defence against infection and stress. Further, we found that loss of Apc lead to a complete loss of response to IL-22
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| |
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| Overall design |
Transcriptome profiling (RNA-seq) was performed on Wild-type (WT) and Adenomatous Polyposis Coli (APC) mutant small intestinal epithelial organoids untreated or treated with IL-22 for 3 hours. 3 independent biological replicates were used for each group.
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| |
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| Contributor(s) |
Swamy M, Chen Y |
| Citation(s) |
31770366 |
| |
| Submission date |
Oct 24, 2019 |
| Last update date |
Jan 08, 2020 |
| Contact name |
Mahima Swamy |
| E-mail(s) |
m.swamy@dundee.ac.uk
|
| Organization name |
University of Dundee
|
| Department |
MRC Protein Phosphorylation and Ubiquitylation Unit
|
| Street address |
Dow Street
|
| City |
Dundee |
| State/province |
Angus |
| ZIP/Postal code |
DD1 5EH |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA579334 |
| SRA |
SRP226828 |
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