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| Status |
Public on Mar 01, 2020 |
| Title |
Targeting CD36-PPARĪ² signaling-mediated metabolic adaptation in intratumoral Tregs primes tumors for PD-1 blockade |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Though high frequency of Tregs in tumor obstructs anti-tumor immune responses, systemic depletion of Tregs caused severe autoimmune disease in mice model. Therefore, specifically targeting intratumoral Tregs is direly needed to fight against tumor. Our study provides a novel approach to target intratumoral Tregs to enhance anti-tumor immunotherapy.
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| Overall design |
The goals of this study are to use NGS to perform transcriptome profiling (RNA-seq) to find the changes of the global gene expression programs among Tregs from different tissues including spleen, lymph node, and tumor. Comparison is also done between Tregs isolated from WT (FoxP3-YFPCre+/+) tumor and KO (CD36floxed FoxP3-YFPCre+/-). Total RNA was extracted and submitted to RNA-seq
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| Contributor(s) |
Wang H, Ho P |
| Citation(s) |
32066953 |
| |
| Submission date |
Oct 24, 2019 |
| Last update date |
May 31, 2020 |
| Contact name |
Ping-Chih Ho |
| E-mail(s) |
ping-chih.ho@unil.ch
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| Organization name |
University of Lausanne
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| Department |
Department of Fundamental Oncology
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| Lab |
Immunometabolic regulation
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| Street address |
Ch. des Boveresses 155
|
| City |
Epalinges |
| State/province |
Vaud |
| ZIP/Postal code |
1066 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA579319 |
| SRA |
SRP226818 |
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