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| Status |
Public on Oct 22, 2019 |
| Title |
Deregulated Adhesion Program in Palatal Keratinocytes of Orofacial Cleft Patients |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Orofacial clefts (OFCs) are the most frequent craniofacial birth defects. An orofacial cleft (OFC) occurs as a result of deviations in palatogenesis. Cell proliferation, differentiation, adhesion, migration and apoptosis are crucial in palatogenesis. We hypothesized that deregulation of these processes in oral keratinocytes contributes to OFC. We performed microarray expression analysis on palatal keratinocytes from OFC and non-OFC individuals. Principal component analysis showed a clear difference in gene expression with 24 and 17% for the first and second component respectively. In OFC cells, 228 genes were differentially expressed (p<0.001). Gene ontology analysis showed enrichment of genes involved in β1 integrin-mediated adhesion and migration, as well as in P-cadherin expression. A scratch assay demonstrated reduced migration of OFC keratinocytes (343.6 ± 29.62 μm) vs. non-OFC keratinocytes (503.4 ± 41.81 μm, p<0.05). Our results indicate that adhesion and migration are deregulated in OFC keratinocytes, which might contribute to OFC pathogenesis.
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| Overall design |
We performed microarray expression analysis and QPCR on palatal keratinocytes from OFC and non-OFC individuals. In addition a functional scratch assay was performed.
Microarray expression study, cultured keratinocytes, QPCR, scratch assay
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| Contributor(s) |
Mammadova A, Carels C, Zhou J, Gilissen C, Helmich M, Bian Z, Zhou H, Von den Hoff J |
| Citation(s) |
31652793 |
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| Submission date |
Oct 21, 2019 |
| Last update date |
Jun 14, 2022 |
| Contact name |
Jo Huiqing Zhou |
| E-mail(s) |
jo.zhou@radboudumc.nl
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| Organization name |
Radboud University
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| Street address |
Geert Grooteplein 26/28
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| City |
Nijmegen |
| ZIP/Postal code |
6525GA |
| Country |
Netherlands |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA578774 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE139222_RAW.tar |
47.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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