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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 19, 2020 |
| Title |
Regulating lineage progression of cortical neural stem cells by extrinsic signaling molecule SHH [bulk RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose:To asses changes in gene expression profiles from the P0 wild type littermate controls cortex, hGFAP-Cre, SmoM2f/+ mice cortex and ShhN IUE (ShhN palsmid were electroporated to wild type mice cortex at E13.5 and the cortex were dissected at P0) mice.
Methords:Total RNA was isolated and sequenced using an illumina high-seq 2500. Raw data was analyzed using TopHat. Genes were considered significantly changed whicn perform fold-change>=2 and P value <= 0.05
Results: Compared to controls, 2466 genes were significantly down-regulated, and 5289 genes were up regulated in the RNA expression level of the SmoM2 mice; 3492 genes were significantly down-regulated, and 3577 genes were up regulated in the RNA expression level of the cortex of ShhN IUE mice.
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| Overall design |
Cortex RNA profiles of P0 Wild type mice brain, ShhN IUE mice brain and hGFAP-Cre, SmoM2f/+ mice brain
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| Contributor(s) |
Zhang Y, Liu G, Guo T, Liang XG, Du H, Yang L, Bhaduri A, Li X, Xu Z, Zhang Z, Li Z, He M, Tsyporin J, Kriegstein AR, Rubenstein JL, Yang Z, Chen B, Yang Z, Chen B |
| Citation(s) |
32234482 |
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| Submission date |
Oct 15, 2019 |
| Last update date |
May 20, 2020 |
| Contact name |
Guoping Liu |
| E-mail(s) |
gpliu@fudan.edu.cn
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| Phone |
18521006300
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| Organization name |
Fudan University
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| Street address |
138 Yi Xue Yuan Road
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| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE140817 |
Cortical Neural Stem Cell Lineage Progression Is Regulated by Extrinsic Signaling Molecule Sonic Hedgehog |
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| Relations |
| BioProject |
PRJNA577670 |
| SRA |
SRP225731 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE138878_all.counts.WT_vs_ShhN_IUE_all.txt.gz |
10.6 Mb |
(ftp)(http) |
TXT |
| GSE138878_all.counts.WT_vs_hGFAP-cre_SmoM2_all.txt.gz |
10.8 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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