|
| Status |
Public on Dec 11, 2008 |
| Title |
Response of p53 isogenic colorectal cancer panel to 12 Gy gamma irradiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Though targeted homologous recombination, we developed a panel of matched colorectal cancer cell lines that differ only with respect to their endogenous TP53 status. We then used these lines to define the genes whose expression was altered following DNA damage induced by ionizing radiation. Transcriptome analyses revealed a consistent upregulation of polo-like kinase I (PLK1) as well as other genes controlling the G2/M transition in the cells whose TP53 genes were inactivated compared to those with wild type (wt ) TP53 genes.
Keywords: Genetic Modification, Stress Response
|
| |
|
| Overall design |
Two-condition experiment, with or without 12 Gy gamma radiation; these conditions are on the same array. No replicates included
|
| |
|
| Contributor(s) |
Pagliarini RA |
| Citation(s) |
19225112 |
| |
| Submission date |
Dec 09, 2008 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Raymond Anthony Pagliarini |
| Organization name |
NIBRI
|
| Department |
Oncology
|
| Street address |
250 Massachusetts Ave. Room 3C-292
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
|
| Samples (13)
|
| GSM349751 |
HCT116 p53 WT colorectal cancer cells_12 Gy gamma radiation |
| GSM349752 |
HCT116 p53 WT HETEROZYGOUS colorectal cancer cells_12 Gy gamma radiation |
| GSM349753 |
HCT116 p53 NULL Colorectal cancer cells_12 Gy gamma radiation |
| GSM349754 |
HCT116 p53 MUT HETEROZYGOUS Colorectal cancer cells_12 Gy gamma radiation |
| GSM349755 |
HCT116 p53 MUT Colorectal cancer cells_12 Gy gamma radiation |
| GSM349756 |
RKO p53 WT Colorectal cancer cells_12 Gy gamma radiation |
| GSM349757 |
RKO p53 WT HETEROZYGOUS Colorectal cancer cells_12 Gy gamma radiation |
| GSM349758 |
RKO p53 NULL Colorectal cancer cells_12 Gy gamma radiation |
| GSM349759 |
DLD-1 p53 MUT Colorectal cancer cells_12 Gy gamma radiation |
| GSM349760 |
DLD-1 p53 WT Colorectal cancer cells_12 Gy gamma radiation |
| GSM349761 |
SW48 p53 WT Colorectal cancer cells_12 Gy gamma radiation |
| GSM349762 |
SW48 p53 WT HETEROZYGOUS Colorectal cancer cells_12 Gy gamma radiation |
| GSM349763 |
SW48 p53 NULL Colorectal cancer cells_12 Gy gamma radiation |
|
| Relations |
| BioProject |
PRJNA110357 |
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