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| Status |
Public on Nov 12, 2020 |
| Title |
Comparative RNAseq of Mouse Parp3+/+ and Parp3-/- Neural Stem Progenitor Cells (NPSC) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To identify the biological pathways underlying impaired differentiation of Parp3-deficient NPSC to astrocytes, RNAseq of the Parp3+/+ and Parp3-/- astrocytes were performed 4 days upon differentiation. Briefly, NPSC were isolated from the forebrains of Parp3+/+ and Parp3-/- 8-day-old mice. Viable single cells were induced to differentiate into astrocytes for 4 days and samples were collected for total RNA isolation and RNA sequencing.
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| Overall design |
The experiment includes 3 independent differentiations (biological replicates) of Parp3+/+ and Parp3-/- NPSC to 4 days astrocytes. The samples represent 3 Parp3+/+ 4d-astrocytes and 3 Parp3-/- 4d-astrocytes.
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| |
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| Contributor(s) |
Dantzer F |
| Citation(s) |
33159039 |
| |
| Submission date |
Oct 13, 2019 |
| Last update date |
Nov 12, 2020 |
| Contact name |
Françoise DANTZER |
| E-mail(s) |
francoise.dantzer@unistra.fr
|
| Phone |
+33368854707
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| Organization name |
CNRS
|
| Department |
UMR7242
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| Lab |
Biotechnology and Cell Signaling
|
| Street address |
300 boulevard S. Brant
|
| City |
Illkirch |
| ZIP/Postal code |
67412 |
| Country |
France |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA577304 |
| SRA |
SRP225462 |
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