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| Status |
Public on Jul 27, 2020 |
| Title |
Gene expression analysis from placenta of Cornelia de Lange Syndrome (CdLS) mouse models |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
CdLS is caused by mutaion in cohesin and associated proteins, such as Nipbl and Hdac8. We performed RNA seq analysis from Nipbl+/- and Hdac8-/0 placenta with their respective wild type control at 14.5 dpc, to identify molecular pathways affected. To understand the effect of protein kinase R (PKR) on gene expression we also performed RNA seq analysis from Nipbl+/- and Hdac8-/0 placenta lacking PKR.
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| Overall design |
Total RNA was isolated using Trizol method and cDNA was prepatred using TruSeq Stranded Total RNA LT kit (RS-122-2302) for Illumina sequencing.
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| Contributor(s) |
Singh VP, Gerton JL |
| Citation(s) |
32800293 |
| |
| Submission date |
Oct 10, 2019 |
| Last update date |
Jul 11, 2022 |
| Contact name |
Madelaine Gogol |
| Organization name |
Stowers Institute
|
| Department |
Computational Biology Core
|
| Street address |
1000 E. 50th Street
|
| City |
Kansas City |
| State/province |
MO |
| ZIP/Postal code |
64110 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (32)
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| Relations |
| BioProject |
PRJNA576802 |
| SRA |
SRP225047 |
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