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Series GSE138622 Query DataSets for GSE138622
Status Public on Apr 07, 2020
Title Effect of Ephrin-A5 DNA Nanocalipers stimulation of EphA2 receptor signaling in U3013 cell line
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Ligand binding induces extensive spatial reorganization and clustering of the EphA2 receptor at the cell membrane. It has previously been shown that the nanoscale spatial distribution of ligands modulates EphA2 receptor reorganization, activation and the invasive properties of cancer cells. However, the mechanisms by which cells transduce ligand nanoscale spatial distribution signals have not been elucidated. Here we used DNA origami nanocalipers to control the positions of ephrin-A5 ligands at the nanoscale and investigated the transcriptional responses following ligand binding. Using mRNA sequencing, we determined the transcriptional profiles of glioblastoma cells treated with nanocalipers presenting a single ephrin-A5 dimer or two dimers spaced 14, 40 or 100 nm apart. We observed divergent transcriptional responses to ephrin-A5 nano-organization, with ephrin-A5 dimers spaced 40 or 100 nm apart showing the highest levels of differential expressed genes compared to treatment with n anocalipers that do not present ephrin-A5. These findings show that the nanoscale organization of ephrin-A5 modulates transcriptional responses to EphA2 activation.
Overall design Here, we compare differently spaced Ephrin-A5 dimers on DNA nanocalipers and the effect they have on the U3013 transcriptome when stimulating the EphA2 receptor
Contributor(s) Verheyen T
Citation(s) 32352518
Submission date Oct 09, 2019
Last update date Jun 08, 2020
Contact name Toon Verheyen
Organization name Karolinska Institutet
Department Medical Biochemistry and Biophysics
Lab Teixeira Lab
Street address Tomtebodavägen 16
City Solna
ZIP/Postal code 17165
Country Sweden
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (20)
GSM4114461 NC-Empty_1
GSM4114462 NC-Empty_2
GSM4114463 NC-Empty_3
This SubSeries is part of SuperSeries:
GSE138623 Ephrin-A5 modulates transcriptional responses to EphA2 activation
BioProject PRJNA576597
SRA SRP224927

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE138622_RAW.tar 3.8 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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