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Series GSE138225 Query DataSets for GSE138225
Status Public on Mar 03, 2020
Title Efficient differentiation and purification of human induced pluripotent stem cell-derived endothelial progenitor cells and expansion with the use of inhibitors of ROCK, TGF-β and GSK3β
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Endothelial cells (ECs) and endothelial progenitor cells (EPCs) play crucial roles in maintaining vascular health and hemostasis. Both cell types have been used in regenerative therapy as well as in various in vitro models; however, the properties of primary human ECs and EPCs are dissimilar owing to differences in genetic backgrounds and sampling techniques. Although human induced pluripotent stem cells (hiPSCs) are an alternative cell source of ECs and EPCs, the differentiation and purification processes have not been optimized. Besides, owing to limited expandability, it is difficult to produce these cells in large numbers. Here we report the development of relatively simple differentiation and purification methods for hiPSC-derived EPCs (iEPCs). Furthermore, we discovered that a combination of three small molecules, that is, Y-27632 (a selective inhibitor of Rho-associated, coiled-coil containing protein kinase [ROCK]), A 83-01 (a receptor-like kinase inhibitor of transforming growth factor beta [TGF-β]), and CHIR-99021 (a selective inhibitor of glycogen synthase kinase-3β [GSK3β] that also activates Wnt), dramatically stimulated protein synthesis-related pathways and enhanced the proliferative capacity of iEPCs. These findings will help to establish a supply system of EPCs at an industrial scale.
Overall design Analysis of gene expressions of iEPCs treated with DMSO or Y-27632, A 83-01, and CHIR-99021 (YAC)
Contributor(s) Aoki H, Yamashita M, Hashita T, Ogami K, Hoshino S, Iwao T, Matsunaga T
Citation(s) 32154424
Submission date Oct 01, 2019
Last update date Mar 16, 2020
Contact name HIromasa Aoki
Organization name Nagoya City University
Street address 3-1 Tanabe-dori, Mizuho-ku
City Nagoya
ZIP/Postal code 467-8603
Country Japan
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (2)
GSM4103045 Cont-iEPCs
GSM4103046 YAC-iEPCs
BioProject PRJNA575153
SRA SRP223837

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Supplementary file Size Download File type/resource
GSE138225_Cont-iEPCs_vs_YAC_iEPCs.Differential_analysis_results.xls.gz 1.6 Mb (ftp)(http) XLS
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Raw data are available in SRA
Processed data are available on Series record

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