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Series GSE138144 Query DataSets for GSE138144
Status Public on Jan 14, 2021
Title Hnf4a regulated transcriptome in murine PDAC autochthonous tumors [FFPE tumors]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Pancreatic ductal adenocarcinoma is an aggressive disease with a dismal five-year survival of 5%. Gene expression profiling has been instrumental for subtype classification in cancer, highlighting fundamental differences in tumors at the molecular level. Over the last years, multiple genomics studies have led to the classification of PDAC into two major subtypes: classical and basal-type. The classical subtype expresses higher levels of endodermal lineage specifiers, including HNF4A, GATA6, FOXA2, FOXA3 than the basal-type. The basal-type confers a worse prognosis, raising the possibility that loss of these lineage specifiers might enhance the malignant potential of PDAC. We found that the lineage specifier HNF4a plays a key role in maintaining a transcriptional network that characterizes the classical subtype, restraining growth in different PDAC models. Additionally, we demonstrated that HNF4a controls PDAC cell identity and proliferation, and represses the expression of SIX family members, two mesodermal lineage specifiers highly expressed in basal-type.
Overall design The study was designed to compare the expression profiles of Hnf4a-positive pancreatic ductal adenocarcinomas with tumors in which Hnf4a was deleted at the time of initiation. RNA was isolated from aniline blue-stained FFPE sections of micro-dissected autochthonous tumors.
Contributor(s) Camolotto SA, Belova VK, Torre-Healy L, Stubben C, Moffit R, Snyder EL
Citation(s) 32826305
Submission date Sep 30, 2019
Last update date May 16, 2022
Contact name Eric Snyder
Organization name University of Utah, Huntsman Cancer Institute
Department Pathology
Street address 2000 Circle of Hope Drive
City Salt Lake City
State/province UT
ZIP/Postal code 84103
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (12)
GSM4100948 14224X1 ES912P
GSM4100949 14224X2 HC673P
GSM4100950 14224X3 HC494P
This SubSeries is part of SuperSeries:
GSE138145 Hnf4a regulated transcriptome
BioProject PRJNA574924
SRA SRP223683

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE138144_counts_FFPE.txt.gz 606.1 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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