|
| Status |
Public on Aug 24, 2020 |
| Title |
High-throughput capture of cis-rearrangement and trans-rearrangement sequence [LAM-HTGTS] |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
Cell cycle is a major determinant of DNA double-strand break (DSB) repair pathway choice with homologous recombination (HR) that is most active in S phase cells and non-homologous end-joining (NHEJ) that dominates in G1 phase cells. A third less well-defined mechanism, 'alternative end-joining', has been shown to promote error-prone repair in NHEJ- or HR-deficient cells. Here, we have used a physiologic system of NHEJ-mediated genomic rearrangements induced by the site-specific RAG1/2 endonuclease in G1 cells to investigate the fate of unrepaired G1 DSBs upon entry into the cell cycle. We show that, in the absence of XRCC4, alternative end-joining rescues RAG-induced DSB repair and promotes chromosome translocations upon G1 cell cycle exit.
|
| |
|
| Overall design |
We performed linear amplification-mediated high-throughput genome-wide sequencing (LAM-HTGTS) on mouse v-Abl/Bcl2 immortalized pro-B cell lines which included genotypes WT, Xrcc4-/-, p53-/- and Xrcc4-/- p53-/-. Igk-J4 primer was used as bait to capture cis-rearrangement sequence within Igk loci and trans-rearrangement sequence with whole genome-wide. The captured of cis- and trans-rearrangement sequences with Igk-J4 were analyzed and compared among cycling cells, v-Abl kinase inhibitor treated and released cells.
*Please note that each wig processed data was generated from all replicates together and is linked to the corresponding rep1 sample records.
|
| |
|
| Contributor(s) |
Yu W, Lescale C, Deriano L |
| Citation(s) |
33067475 |
| |
| Submission date |
Sep 30, 2019 |
| Last update date |
Oct 27, 2020 |
| Contact name |
Ludovic Deriano |
| E-mail(s) |
ludovic.deriano@pasteur.fr
|
| Organization name |
Institut Pasteur
|
| Department |
Immunology
|
| Lab |
Lymphocyte Development and Oncogenesis
|
| Street address |
25-28 Rue du Docteur Roux
|
| City |
Paris |
| ZIP/Postal code |
75015 |
| Country |
France |
| |
|
| Platforms (1) |
|
| Samples (32)
|
|
| This SubSeries is part of SuperSeries: |
| GSE138137 |
High-throughput sequencing of RAG induced re-arrangements |
|
| Relations |
| BioProject |
PRJNA574913 |
| SRA |
SRP223675 |
Less...
More...