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| Status |
Public on Oct 28, 2020 |
| Title |
Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Diabetic db/db Mice Islets Transcriptomes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Circular RNAs constitute an extensive fraction of the mammalian transcriptome.In this study, circular RNAs dysregulated in the islets of diabetic db/db mice were identified by high-throughput RNA sequencing. More than 5000 circRNAs were detected using RNA sequencing, indicating that circular transcripts were abundant in islets. Among them, we found that circ-Tulp4 showed significant downregulation in diabetic mouse islet cells. Further experiments indicated that modulating the expression of circ-Tulp4 in β cell lines desensitized β-cells to lipotoxicity.
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| Overall design |
Islet circRNAs and mRNA profiling in db/db and db/m control mice were generated by deep sequencing, in triplicate, using Illumina Hiseq.
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| Contributor(s) |
Wu L, Xiong L |
| Citation(s) |
33064661 |
| |
| Submission date |
Sep 27, 2019 |
| Last update date |
Oct 28, 2020 |
| Contact name |
Liting Wu |
| E-mail(s) |
wult5@mail2.sysu.edu.cn
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| Organization name |
The First Affiliated Hospital of Sun Yat-sen University
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| Department |
Department of Endocrinology and Metabolism
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| Street address |
Zhongshan Road 2
|
| City |
Guangzhou |
| ZIP/Postal code |
510080 |
| Country |
China |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA574600 |
| SRA |
SRP223504 |
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