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Series GSE137970 Query DataSets for GSE137970
Status Public on Sep 26, 2019
Title Next Generation Sequencing Facilitates Quantitative Analysis of TFH2 cell Transcriptomes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Emergence of IgE antibodies that bind allergens with high affinity is highly correlated with the severity of anaphylaxis. However, the underlying molecular mechanisms by which high-affinity IgE is generated remain poorly understood. We discovered that IL-13-producing T follicular helper (TFH) cells, which we designated as TFH2 cells, are generated from conventional TH2 cells by antigen sensitization. TFH2 cells have a unique cytokine profile (IL-4+IL-5-IL-13+IL-21+) and co-express the TFH and TH2 master transcriptional regulators, BCL-6 and GATA3.
Overall design Naïve CD4T, TH2, or TFH cells of mice were sorted and analyzed the transcriptome using Illumina HiSeq1500.
Contributor(s) Kubo M
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Submission date Sep 25, 2019
Last update date Sep 28, 2019
Contact name Kosuke Miyauchi
Organization name RIKEN Center for Integrative Medical Sciences
Street address 1-7-22 Suehiro-sho
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (30)
GSM4095363 Sample 1_Naïve CD4T
GSM4095364 Sample 2_TH2
GSM4095365 Sample 3_TFH
BioProject PRJNA574058
SRA SRP223238

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE137970_RAW.tar 8.8 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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