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| Status |
Public on Oct 31, 2019 |
| Title |
DNA methylation in airway epithelial cells isloated by pronase and bronchial brush from individuals with and without asthma |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
|
| Summary |
The airway epithelium forms the interface between the inhaled environment and the lung. The airway epithelium is dysfunctional in asthma and epigenetic mechanisms are considered a contributory factor. We hypothesised that the DNA methylation profiles of cultured primary airway epithelial cells (AECs) would differ between cells isolated from individuals with asthma (n=17) versus those without asthma (n=16). AECs were isolated from patients by two different isolation techniques; pronase digestion (9 non-asthmatic, 8 asthmatic) and bronchial brushings (7 non-asthmatic and 9 asthmatic). DNA methylation was assessed using an Illumina Infinium HumanMethylation450 BeadChip array. DNA methylation of AECs clustered by isolation technique and linear regression identified 111 CpG sites differentially methylated between isolation techniques in healthy individuals. As a consequence, the effect of asthmatic status on DNA methylation was assessed within AEC samples isolated using the same technique. In pronase isolated AECs, 15 DNA regions were differentially methylated between asthmatics and non-asthmatics. In bronchial brush isolated AECs, 849 differentially methylated DNA regions were identified with no overlap to pronase regions. In conclusion, regardless of cell isolation technique, differential DNA methylation was associated with asthmatic status in AECs, providing further evidence for aberrant DNA methylation as a signature of epithelial dysfunction in asthma.
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| Overall design |
asthma vs no asthma using two different isolation techniques
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| |
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| Contributor(s) |
Clifford RL |
| Citation missing |
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| |
| Submission date |
Sep 19, 2019 |
| Last update date |
Nov 02, 2019 |
| Contact name |
Rachel Clifford |
| E-mail(s) |
r.clifford@nottingham.ac.uk
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| Organization name |
University of Nottingham
|
| Street address |
Biodiscovery Institute 3
|
| City |
nottingham |
| ZIP/Postal code |
NG7 2RD |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (33)
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| Relations |
| BioProject |
PRJNA566386 |
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