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Series GSE137028 Query DataSets for GSE137028
Status Public on Apr 17, 2020
Title Transcriptomic profiling of microglia and astrocytes throughout aging
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose:The goal of this study are to understand the role of miceoglia and astrocyte in both physiological and pathological conditions. Methods: Microglia and astrocyte were isolated from 5 time points WT/APP-PS1 mice in triplicate. mRNA were generated by SMART seq2 using Illumina Novaseq platform. Conclusions: Using DESeq2 to sequence polyA-selected mRNAs from microglia and astrocytes isolated from whole brain. We mapped >85% of reads for all of our sample. We found good reproducibility between the replicates
Overall design We performed RNA-seq in microglia and astrocytes freshly isolated from Wild-type(WT)/APP-PS1(AD) mice brains across the lifespan to analyze the longitudinal genes expression profile.
Contributor(s) Pan J, Wan J
Citation(s) 32238175
Submission date Sep 06, 2019
Last update date Apr 17, 2020
Contact name jie pan
Phone 18666886732
Organization name Shenzhen Peking University – The Hong Kong University of Science and Technology Medical Center
Street address Lian Hua Lu 1120, FuTian District Guangdong
City shenzhen
ZIP/Postal code 518000
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (60)
GSM4065891 WT2m1M
GSM4065892 WT2m2M
GSM4065893 WT2m3M
BioProject PRJNA564299
SRA SRP220678

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Supplementary file Size Download File type/resource
GSE137028_gene_fpkm.xls.gz 8.5 Mb (ftp)(http) XLS
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Processed data are available on Series record

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