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| Status |
Public on Apr 17, 2020 |
| Title |
Transcriptomic profiling of microglia and astrocytes throughout aging |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose:The goal of this study are to understand the role of miceoglia and astrocyte in both physiological and pathological conditions. Methods: Microglia and astrocyte were isolated from 5 time points WT/APP-PS1 mice in triplicate. mRNA were generated by SMART seq2 using Illumina Novaseq platform. Conclusions: Using DESeq2 to sequence polyA-selected mRNAs from microglia and astrocytes isolated from whole brain. We mapped >85% of reads for all of our sample. We found good reproducibility between the replicates
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| Overall design |
We performed RNA-seq in microglia and astrocytes freshly isolated from Wild-type(WT)/APP-PS1(AD) mice brains across the lifespan to analyze the longitudinal genes expression profile.
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| Contributor(s) |
Pan J, Wan J |
| Citation(s) |
32238175 |
| Submission date |
Sep 06, 2019 |
| Last update date |
Apr 17, 2020 |
| Contact name |
jie pan |
| E-mail(s) |
panj@bjmu.edu.cn
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| Phone |
18666886732
|
| Organization name |
Shenzhen Peking University – The Hong Kong University of Science and Technology Medical Center
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| Street address |
Lian Hua Lu 1120, FuTian District Guangdong
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| City |
shenzhen |
| ZIP/Postal code |
518000 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (60)
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| Relations |
| BioProject |
PRJNA564299 |
| SRA |
SRP220678 |
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