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Series GSE136870 Query DataSets for GSE136870
Status Public on Jan 24, 2020
Title Mouse ESCs (ES-D3) with Ash2l depletion
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We showed a novel mechanism in which Ash2l directly binds to super-enhancers of several stemness genes to regulate pluripotency and self-renewal in pluripotent stem cells. Ash2l recruits Oct4/Sox2/Nanog (OSN) to form Ash2l/OSN complex at the super-enhancers of Jarid2, Nanog, Sox2, and Oct4, and further drives enhancer activation, upregulation of stemness genes, and maintains the pluripotent circuitry. Ash2l knockdown abrogates the OSN recruitment to all super-enhancers and further hinders the enhancer activation.
Overall design Mouse ESCs (ES-D3) were infected with lentivirus containing either control or shRNA targeting Ash2l sequence. DNA was then extracted from both samples and subjected to ChIP-seq analysis.
Contributor(s) Tsai P, Chiou S
Citation(s) 31555818
Submission date Sep 04, 2019
Last update date Jan 24, 2020
Contact name Pinghsing Tsai
Organization name National Yang-Ming university
Department Pharmacology
Street address Rm. 719, 7F., No.322, Sec. 2, Shipai Rd., Beitou D
City Taipei
State/province Taiwan
ZIP/Postal code 11267
Country Taiwan
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (13)
GSM4060028 shCtrl-Oct4_ChIPSeq
GSM4060029 shAsh2l-Oct4_ChIPSeq
GSM4060030 shCtrl-Sox2_ChIPSeq
BioProject PRJNA563897
SRA SRP220339

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Supplementary file Size Download File type/resource
GSE136870_Antibody-information.xlsx 9.2 Kb (ftp)(http) XLSX
GSE136870_RAW.tar 1.4 Mb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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