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| Status |
Public on Jan 24, 2020 |
| Title |
Mouse ESCs (ES-D3) with Ash2l depletion |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We showed a novel mechanism in which Ash2l directly binds to super-enhancers of several stemness genes to regulate pluripotency and self-renewal in pluripotent stem cells. Ash2l recruits Oct4/Sox2/Nanog (OSN) to form Ash2l/OSN complex at the super-enhancers of Jarid2, Nanog, Sox2, and Oct4, and further drives enhancer activation, upregulation of stemness genes, and maintains the pluripotent circuitry. Ash2l knockdown abrogates the OSN recruitment to all super-enhancers and further hinders the enhancer activation.
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| Overall design |
Mouse ESCs (ES-D3) were infected with lentivirus containing either control or shRNA targeting Ash2l sequence. DNA was then extracted from both samples and subjected to ChIP-seq analysis.
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| Contributor(s) |
Tsai P, Chiou S |
| Citation(s) |
31555818 |
| |
| Submission date |
Sep 04, 2019 |
| Last update date |
Jan 24, 2020 |
| Contact name |
Pinghsing Tsai |
| Organization name |
Taipei Veterans General Hospital
|
| Street address |
Rm. 719, 7F., No.322, Sec. 2, Shipai Rd., Beitou D
|
| City |
Taipei |
| State/province |
Taiwan |
| ZIP/Postal code |
11267 |
| Country |
Taiwan |
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| Platforms (1) |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA563897 |
| SRA |
SRP220339 |
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