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Series GSE136862 Query DataSets for GSE136862
Status Public on Oct 18, 2019
Title Genome-wide mapping of oxidative DNA damage via engineering of 8-oxoguanine DNA glycosylase
Organism Mus musculus
Experiment type Other
Summary 7,8-dihydro-8-oxoguanine (OG) is one of the major DNA oxidative damages, which has been concerned by researchers for its potential of mutagenesis and epigenetic regulation. Genome-wide mapping of oxidative DNA damage is of great value for understanding the biological impact of these modifications. Here we present a novel appoach to profile guanine oxidation in the mouse genome. Through structure-guided mutagenesis, we've successufully engineered 8-oxoguanine DNA glycosylase into a useful tool for OG detection. By enrichment of glycosylase-DNA complex, thousands of OG peaks were identified in the mouse genome.
Overall design Enrichment procedure was perform on mouse embryonic fibroblasts (MEFs) with two biological replicates. Genomic samples without any treatment were denoted as input; samples enriched by engineered glycosylase were denoted as enrich; samples pretreated with Fpg to remove oxidative damages before enrichment were denoted as control.
Contributor(s) Fang Y, Zou P
Citation(s) 31618020
Submission date Sep 04, 2019
Last update date Feb 20, 2020
Contact name Yuxin Fang
Phone 15122297861
Organization name Peking University
Street address Yiheyuan Road No.5
City Beijing
State/province Beijing
ZIP/Postal code 100871
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (6)
GSM4059846 MEF_input_1
GSM4059847 MEF_enrich_1
GSM4059848 MEF_control_1
BioProject PRJNA563884
SRA SRP220329

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE136862_MEF_peaks_HC.bed.gz 13.8 Kb (ftp)(http) BED
GSE136862_RAW.tar 1.0 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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