|
| Status |
Public on Jan 17, 2020 |
| Title |
Anti-inflammatory properties of low dose epirubicin on human macrophages |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Low-dose epirubicin at non-cytotoxic doses down regulated NLRP3 inflammasome components and reduced the release of proinflammatory cytokines.
These anti-inflammatory effects were a result of global transcriptional suppression of LPS-dependent genes.
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| |
|
| Overall design |
THP-1 cells were differentiated to macrophages and cells were treated with or without epirubicin (0.25 μg/ml) for 24h and with or without LPS (100 ng/ml) for three hours resulting in four treatment conditions (1. -EPI/- LPS; 2. –EPI/+LPS; 3. +EPI/-LPS; 4. +EPI/+LPS). RNA was isolated and microarray analysis was performed by a commercial transcriptomics provider. Expression analysis was performed using Transcriptome analysis concole (TAC).
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| |
|
| Contributor(s) |
Stengel S, Bruns T |
| Citation(s) |
31905600 |
| |
| Submission date |
Sep 03, 2019 |
| Last update date |
Mar 09, 2020 |
| Contact name |
Sven Stengel |
| E-mail(s) |
Stengel2010@googlemail.com
|
| Organization name |
Jena University Hospital
|
| Department |
Clinic for Internal Medicine IV
|
| Lab |
Gastroenterology, Hepatology and Infectiology
|
| Street address |
Am Klinikum 1
|
| City |
Jena |
| ZIP/Postal code |
07747 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL24324 |
[Clariom_S_Human_HT] Affymetrix Clariom S Assay HT, Human (Includes Pico Assay) |
|
| Samples (8)
|
| GSM4057493 |
THP1; EPI 0 ug/ml; LPS 100 ng/ml; dup2 |
| GSM4057494 |
THP1; EPI 0.25 ug/ml; LPS 0 ng/ml; dup1 |
| GSM4057495 |
THP1; EPI 0.25 ug/ml; LPS 0 ng/ml; dup2 |
| GSM4057496 |
THP1; EPI 0.25 ug/ml; LPS 100 ng/ml; dup1 |
| GSM4057497 |
THP1; EPI 0.25 ug/ml; LPS 100 ng/ml; dup2 |
|
| Relations |
| BioProject |
PRJNA563607 |
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