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Series GSE136694 Query DataSets for GSE136694
Status Public on Jun 01, 2020
Title Transcription Factor Tox2 is required for Follicular Helper T Cells differentiation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Follicular helper T (Tfh) cells are crucial for formation of B cell germinal center (GC) and long-term production of antigen-specific antibodies. The molecular mechanism underlying Tfh cell differentiation, however, still remains largely unknown. Here, we show that the transcription factor Thymocyte selection-associated high mobility group box family member 2 (Tox2) is important for Tfh cell development. Tox2 deficiency led to impaired Tfh cell differentiation and subsequently reduced GC formation. Consistently, ectopic expression of Tox2 promoted Tfh cell differentiation. Mechanistically, we found that Tox2 regulated the expression of Ascl2, an initiator in driving CXCR5 transcription in Tfh cells; Tox2 deficiency caused the decreased expression of Ascl2. Restoration of Ascl2 expression in Tox2 deficient CD4+ T cells rescued the defect in Tfh cell differentiation. Our study suggests that Tox2 is an important transcription factor for Tfh cell development.
Overall design WT OT-II or Tox2-/- SMARTA mice were transferred into Ly5.1 mice, followed by LCMV infection. Spleens were harvested 7 dpi to sort. For RNAseq, We have four samples, wt-Tfh, ko-Tfh, wt-nonTfh and ko-nonTfh and 2 replicates for Tfh sample.
Contributor(s) Wang H, Tang J, Deng X
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Submission date Aug 30, 2019
Last update date Jun 01, 2020
Contact name Jinyi Tang
Phone 17721241521
Organization name IPS
Lab Wang Haikun
Street address Yueyang road 320
City Shanghai
ZIP/Postal code 200031
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (6)
GSM4055568 RNA_wt-Tfh_1
GSM4055569 RNA_wt-Tfh_2
GSM4055570 RNA_ko-Tfh_1
BioProject PRJNA563072
SRA SRP219955

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE136694_TFH_raw_transcript_abundance.txt.gz 303.6 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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