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Series GSE136364 Query DataSets for GSE136364
Status Public on Jul 31, 2023
Title Testing data
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Microglia are important immune cells in the central nervous system (CNS). Dysfunctions of gene-deficient microglia contribute to the development and progression of multiple CNS diseases. Microglia replacement by nonself cells has been proposed to treat microglia associated disorders. However, most of attempts are failed due to low replacement efficiencies, such as with the traditional bone marrow transplantation approach. In this study, we develop three efficient strategies for microglia replacement: microglia replacement by bone marrow transplantation (mrBMT), microglia replacement by peripheral blood (mrPB) and microglia replacement by microglia transplantation (mrMT). mrBMT and mrPB allow microglia-like cells to efficiently replace resident microglia in the whole CNS. On the other hand, mrMT achieves microglia replacement in brain regions of interest. In summary, the present study offers effective tactics for microglia replacement with diverse application scenarios, which potentially opens up a window on treating microglia-associated CNS disorders.
Overall design Single-cell RNA-seq for normal microglia and microglia (or microglia-like cells) from mrMT, mrBMT and mrPB. Cells were collected 3 months after transplantation.
Contributor(s) Peng B, Rao Y
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Submission date Aug 26, 2019
Last update date Sep 14, 2023
Contact name Bo Peng
Organization name Fudan University
Department Institute for Translational Brain Research
Lab Bo Peng Lab (aka PB Lab)
Street address 138 Yixueyuan Road
City Shanghai
ZIP/Postal code 200032
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (4)
GSM4047448 Normal microglia
GSM4047449 Mr MT microglia
GSM4047450 Mr BMT microglia-like cells
BioProject PRJNA562298
SRA SRP219674

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE136364_RAW.tar 938.0 Mb (http)(custom) TAR (of TAR)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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