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| Status |
Public on Aug 22, 2019 |
| Title |
Gene expression profiling in lung and breast cancer cells treated by Bloom-specific siRNAs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
A549 and MDA-MB-231 cells were transfected with the Bloom-specific siRNA. Total RNA was extracted using Trizol at 48 hours after transfection. RNA-SEQ was carried out to profile the gene expression in both culture conditions.
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| |
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| Overall design |
The goal of this experiment was to profie the genes that are regulated by Bloom (BLM RecQ like helicase) in cancer cells. Gene expression was probed globally using RNA-SEQ.
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| Contributor(s) |
Shan B, Alsager S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 21, 2019 |
| Last update date |
Aug 24, 2019 |
| Contact name |
Bin Shan |
| E-mail(s) |
bin.shan@wsu.edu
|
| Phone |
015093587813
|
| Organization name |
Washington State University
|
| Department |
Biomedical Sciences
|
| Street address |
412 E Spokane Falls Blvd
|
| City |
Spokane |
| State/province |
Washington |
| ZIP/Postal code |
99202 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA561309 |
| SRA |
SRP218977 |
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