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| Status |
Public on Nov 04, 2020 |
| Title |
Single cell RNA sequencing reveals functional heterogeneity and sex differences of glioma-associated brain macrophages |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Single cell RNA-seq analysis of CD11b+ cells sorted from murine syngeneic gliomas, indicating distinct activity of microglia, infiltrating monocytes/macrophages and CNS border associated macrophages. It demonstrates a molecular heterogeneity in the innate immune response in gliomas. Differently expressed genes were identified in activated microglia from glioma-bearing mice of different sex, and profound overexpression of the MHCII genes was observed in male microglial cells.
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| Overall design |
Single cell RNA sequencing analysis of 4 groups: female control (2 samples), female tumor (14 days post implantation, 2 samples), male control (2 samples), male tumor (14 days post implantation, 2 samples).
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| Contributor(s) |
Ochocka N, Segit P, Walentynowicz KA, Wojnicki K, Cyranowski S, Swatler J, Mieczkowski J, Kaminska B |
| Citation(s) |
33608526 |
| |
| Submission date |
Aug 19, 2019 |
| Last update date |
Mar 10, 2021 |
| Contact name |
Jakub Mieczkowski |
| E-mail(s) |
jmieczkowski@jmieczkowski.pl
|
| Organization name |
Medical University of Gdansk
|
| Street address |
Debinki 12
|
| City |
Gdansk |
| ZIP/Postal code |
80-211 |
| Country |
Poland |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA560921 |
| SRA |
SRP218842 |
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