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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 01, 2020 |
| Title |
Assessment of the Mode of Action Underlying the Effects of GenX in Mouse Liver and Implications for Assessing Human Health Risks |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate the potential role of peroxisome proliferator activated receptor–alpha (PPARa) activation in mice, RNA sequencing was conducted on paraffin-embedded liver sections from a 90-day toxicity study of GenX conducted in mice. the TempO-Seq platform was utilized for RNA sequencing data collection. Differentially expressed genes were identified for each treatment group, and gene set enrichment analysis was conducted using gene sets that represent biological processes and known canonical pathways. Significant enrichment of peroxisome signaling was evident in males and females exposed to 0.5 and 5 mg/kg GenX, with no enrichment at 0.1 mg/kg. PPAR signaling and fatty acid metabolism were among the most significantly enriched gene sets, and two gene sets specific to the PPARa subtype were also significantly enriched.
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| Overall design |
Examination of whole transcriptome expression by targeted sequencing in liver tissue from male and female mice treated with varying concentrations of ammonium 2,3,3,3-tetrafluro-2-(heptafluoropropoxy)-propanoate (CASRN 62037-80-3, "GenX").
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| Contributor(s) |
Chappell GA, Thompson CM |
| Citation(s) |
32138627 |
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| Submission date |
Aug 18, 2019 |
| Last update date |
May 07, 2020 |
| Contact name |
Grace Chappell |
| E-mail(s) |
gchappell@toxstrategies.com
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| Organization name |
ToxStrategies, Inc.
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| Department |
Health Sciences
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| Street address |
9390 Research Blvd, Bldg 2, Ste. #100
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| City |
Austin |
| State/province |
TX |
| ZIP/Postal code |
78723 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (40)
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| GSM4038197 |
Liver, control, 104 |
| GSM4038198 |
Liver, control, 105 |
| GSM4038199 |
Liver, control, 151 |
| GSM4038200 |
Liver, control, 152 |
| GSM4038201 |
Liver, control, 153 |
| GSM4038202 |
Liver, control, 154 |
| GSM4038203 |
Liver, control, 155 |
| GSM4038204 |
Liver, GenX, 0.1mg/kg, 201 |
| GSM4038205 |
Liver, GenX, 0.1mg/kg, 202 |
| GSM4038206 |
Liver, GenX, 0.1mg/kg, 203 |
| GSM4038207 |
Liver, GenX, 0.1mg/kg, 204 |
| GSM4038208 |
Liver, GenX, 0.1mg/kg, 205 |
| GSM4038209 |
Liver, GenX, 0.1mg/kg, 251 |
| GSM4038210 |
Liver, GenX, 0.1mg/kg, 252 |
| GSM4038211 |
Liver, GenX, 0.1mg/kg, 253 |
| GSM4038212 |
Liver, GenX, 0.1mg/kg, 254 |
| GSM4038213 |
Liver, GenX, 0.1mg/kg, 255 |
| GSM4038214 |
Liver, GenX, 0.5mg/kg, 301 |
| GSM4038215 |
Liver, GenX, 0.5mg/kg, 302 |
| GSM4038216 |
Liver, GenX, 0.5mg/kg, 303 |
| GSM4038217 |
Liver, GenX, 0.5mg/kg, 304 |
| GSM4038218 |
Liver, GenX, 0.5mg/kg, 305 |
| GSM4038219 |
Liver, GenX, 0.5mg/kg, 351 |
| GSM4038220 |
Liver, GenX, 0.5mg/kg, 352 |
| GSM4038221 |
Liver, GenX, 0.5mg/kg, 353 |
| GSM4038222 |
Liver, GenX, 0.5mg/kg, 354 |
| GSM4038223 |
Liver, GenX, 0.5mg/kg, 355 |
| GSM4038224 |
Liver, GenX, 5mg/kg, 401 |
| GSM4038225 |
Liver, GenX, 5mg/kg, 402 |
| GSM4038226 |
Liver, GenX, 5mg/kg, 403 |
| GSM4038227 |
Liver, GenX, 5mg/kg, 404 |
| GSM4038228 |
Liver, GenX, 5mg/kg, 405 |
| GSM4038229 |
Liver, GenX, 5mg/kg, 451 |
| GSM4038230 |
Liver, GenX, 5mg/kg, 452 |
| GSM4038231 |
Liver, GenX, 5mg/kg, 453 |
| GSM4038232 |
Liver, GenX, 5mg/kg, 454 |
| GSM4038233 |
Liver, GenX, 5mg/kg, 455 |
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| Relations |
| BioProject |
PRJNA560713 |
| SRA |
SRP218692 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE135943_SP0106_GeneCounts_GEO.csv.gz |
1.3 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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