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| Status |
Public on Dec 23, 2019 |
| Title |
Pachytene piRNA Genes Are Rapidly Diverging Among Modern Humans |
| Organisms |
Macaca mulatta; Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
In the fetal mouse testis, PIWI Interacting RNAs (piRNAs) guide PIWI proteins to silence transposons, but after birth, most post-pubertal pachytene piRNAs map to genome uniquely and are thought to regulate genes required for male fertility. In human males, the developmental classes, precise genomic origins, and transcriptional regulation of post-natal piRNAs remain undefined. Here, we demarcate the genes and transcripts that produce post-natal piRNAs in human juvenile and adult testes. As in mouse, A‑MYB in humans drives transcription of both pachytene piRNA precursor transcripts and the mRNAs encoding piRNA biogenesis factors. Although human piRNA genes are syntenic to those in other placental mammals, their sequences are poorly conserved. In fact, pachytene piRNA loci are rapidly diverging even among modern humans. Our findings suggest that during mammalian evolution, pachytene piRNA genes are under fewer selective constraints. We speculate that pachytene piRNA diversity may provide a hitherto unrecognized driver of reproductive isolation.
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| Overall design |
RNA-seq, small RNA-seq and ChIP-seq for human, mouse, and rhesus macaque.
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| Contributor(s) |
Tianxiong Y, Deniz ÖM |
| Citation(s) |
31900453 |
| BioProject |
PRJNA506245 |
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| Submission date |
Aug 13, 2019 |
| Last update date |
Mar 23, 2020 |
| Contact name |
Tianxiong Yu |
| Organization name |
Umass Med
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| Street address |
364 Plantation St
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| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
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| Platforms (3) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL21120 |
Illumina NextSeq 500 (Macaca mulatta) |
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| Samples (72)
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| Relations |
| SRA |
SRP185903 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE135791_RAW.tar |
11.5 Gb |
(http)(custom) |
TAR (of BW, NARROWPEAK, TXT) |
| GSE135791_merged.rpm.txt.gz |
13.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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