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| Status |
Public on Sep 10, 2019 |
| Title |
Laboratory Evolution Experiments Help Identify a Predominant Site of ΔrnhA-dnaA Constitutive Stable DNA Replication Initiation |
| Organism |
Escherichia coli str. K-12 substr. MG1655 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The study evaluated the effect of cSDR on gene expression by comparing the gene expression states in ΔrnhA-dnaA and evolved suppressor mutants
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| |
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| Overall design |
Total RNA was extracted from E.coli populations grown in LB media at exponential phase of growth and RNA-sequencing using Illumina Hi-Seq 2500 platform, with two biological replicates for each sample.
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| |
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| Contributor(s) |
Veetil RT, Seshasayee AS |
| Citation(s) |
32102945 |
| |
| Submission date |
Aug 12, 2019 |
| Last update date |
Mar 16, 2020 |
| Contact name |
Aswin Sai narain Seshasayee |
| E-mail(s) |
amma.reshma@gmail.com
|
| Phone |
08023666059
|
| Organization name |
National Centre for Biological Sciences
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| Department |
Bioinformatics
|
| Lab |
Bugbears
|
| Street address |
GKVK campus Bellary road
|
| City |
Bengaluru |
| State/province |
Karnataka |
| ZIP/Postal code |
560065 |
| Country |
India |
| |
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| Platforms (1) |
| GPL18956 |
Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA559809 |
| SRA |
SRP218086 |
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