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| Status |
Public on Jan 17, 2020 |
| Title |
The Influence of Met Receptor Level on HGF-Induced Glycolytic Reprogramming in Head and Neck Squamous Cell Carcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Head and neck squamous cell carcinomas (HNSCC) are known to overexpress a variety of receptor tyrosine kinases, such as the HGF receptor Met. Like other malignancies, there is a mutual interaction between the tumor cells and surrounding tissues and cells. We hypothesized that activation of HGF/Met signaling in HNSCC might influence glucose metabolism and therefore substantially changes the tumor microenvironment. To determine the effect of HGF, we used three established HNSCC cell lines and flow cytometry, western blot and RNA sequencing. Dynamic changes in glucose metabolism were measured real-time by an Extracellular flux analyzer. As expected, the cell lines exhibited different levels of Met and responded differently to HGF stimulation. As confirmed by RNA sequencing, the level of Met expression is associated with the number of upregulated HGF-dependent genes. Overall, Met stimulation by HGF leads to increased glycolysis, presumably mediated by higher expression of three key enzymes of glycolysis. These effects appear to be stronger in Methigh expressing HNSCC cells. Collectively our data supports the hypothesized role of HGF/Met signaling in metabolic reprogramming of HNSCC.
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| |
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| Overall design |
3 cell lines, of each one an untreated control and one sample treated with 50 ng/ml HGF for 48 hours, 3 replicates
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| Contributor(s) |
Boschert V, Bischler T, Hartmann S |
| Citation(s) |
31940827, 33233528, 39000441 |
| |
| Submission date |
Aug 08, 2019 |
| Last update date |
Aug 08, 2024 |
| Contact name |
Tom Gräfenhan |
| E-mail(s) |
ht-seq.sysmed@uni-wuerzburg.de
|
| Phone |
+49 - 931 - 31 - 80814
|
| Organization name |
University of Wuerzburg
|
| Department |
Core Unit SysMed
|
| Lab |
Raum D15.02.045
|
| Street address |
Josef-Schneider-Str. 2, Bau D15
|
| City |
Würzburg |
| ZIP/Postal code |
97080 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (18)
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| Relations |
| BioProject |
PRJNA559273 |
| SRA |
SRP217801 |
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