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Series GSE13548 Query DataSets for GSE13548
Status Public on May 20, 2009
Title Expression data from human cancer cells treated with UPR modulators under ER stress conditions
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The unfolded protein response (UPR) is a cellular defense mechanism against glucose deprivation, a cell condition that occurs in solid tumors.
A key feature of the UPR is the activation of the transcription program that allows the cell to cope with endoplasmic reticulum (ER) stress. We used micoarrays to show that the UPR transcription program is disrupted by the antitumor macrocyclic compound versipelostatin (VST) and antidiabetic biguanides metformin, buformin and phenformin, depending on cellular glucose availability.

Keywords: stress response, drug response
 
Overall design Total 42 samples were prepared for RNA extraction and hybridization on Affymetrix microarrays.

To induce the UPR, we treated cells (HeLa, HT-29, HT1080, MKN74) for 15 or 18 hours under ER stress conditions by replacing the medium with glucose-free medium or by adding either 2-Deoxy-D-glucose (2DG) or Tunicamycin (TM) to glucose-containing medium. UPR modulators (VST, biguanides or pyrvinium pamoate) were added at various final concentrations immediately after cells were placed in glucose-free medium or just before the chemical stressors were added to glucose-containing culture medium.
 
Contributor(s) Saito S, Tsuruo T, Tomida A
Citation(s) 19435925
Submission date Nov 10, 2008
Last update date Mar 25, 2019
Contact name Akihiro Tomida
E-mail(s) akihiro.tomida@jfcr.or.jp
Phone +81-3-3520-0111
Organization name Japanese Foundation for Cancer Research (JFCR)
Department Cancer Chemotherapy Center
Lab Division of Genome Research
Street address 3-8-31
City Ariake
State/province Koto-ku, Tokyo
ZIP/Postal code 135-8550
Country Japan
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (42)
GSM341386 HeLa cell_normal growth condition_18h
GSM341387 HeLa cell_normal growth condition_15h
GSM341388 HeLa cell_normal growth condition_1μM VST_18h
Relations
BioProject PRJNA110727

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13548_RAW.tar 198.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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