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| Status |
Public on Aug 02, 2022 |
| Title |
Integrin αEβ7+ T cells direct intestinal stem cell fate decision via adhesive signaling |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Intestinal stem cells (ISCs) maintain gut homeostasis by differentiating into different epithelial cell types, which is precisely regulated by a niche of accessory cell types. In the niche, lymphocytes may interact with stem and transient amplifying (TA) cells in the intestinal crypts. However, it is unknown whether and how the lymphocyte- stem/TA cell contacts affect ISC differentiation. Here we discover and mechanistically dissect the role of T cell-stem/TA cell contacts in ISC fate decision. We found T cells are often seen in contact with ISCs and TA cells in the small intestinal crypts, whereas B cell-stem/TA cell interaction was hardly observed. Using single-cell RNA sequencing and immunostaining, we showed that depletion of intestinal lymphocytes resulted in aberrant ISC differentiation in mice, with decreased Paneth and goblet cells, increased enteroendocrine cells and impaired enterocyte maturation. Transferring T cells but not B cells into those mice efficiently restored normal ISC differentiation. Mechanistically, integrin αEβ7 on T cells binds to E-cadherin on ISCs and TA cells. Blocking this adhesion suppressed Wnt signaling in ISCs and TA cells and promoted Notch signaling in TA cells, leading to defective ISC differentiation. In vitro study using purified αEβ7 protein showed consistent effects on the intestinal organoid differentiation. Taken together, our study reveals that the gut-resident integrin αEb7+ T cells regulate the ISC differentiation through adhesion between αEβ7 on T cells and E-cadherin on ISCs and TA cells. The αEβ7-E-cadherin adhesive signaling is critical for the fate decision of ISCs and the maintenance of intestinal homeostasis.
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| Overall design |
Using single-cell RNA-seq of enriched crypt epithelial cells from C57BL/6J WT and integrin β7 knock out (Itgb7-/-) mice small intestines in triplicate, we found that lack of intestinal lymphocytes resulted in aberrant ISC differentiation in mice, with decreased Paneth and goblet cells, increased enteroendocrine cells and impaired enterocyte maturation.
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| Contributor(s) |
Chen J, Chen S |
| Citation missing |
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| Submission date |
Aug 02, 2019 |
| Last update date |
Aug 04, 2022 |
| Contact name |
Chen Shiyang |
| E-mail(s) |
chenshiyang2014@sibcb.ac.cn
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| Organization name |
Shanghai Institute of Biochemistry and Cell Biology (CAS)
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| Lab |
Jianfeng Chen's Lab
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| Street address |
Room 413, New biochemistry building, YueYang Road
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| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA558381 |
| SRA |
SRP217224 |
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