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Series GSE135314 Query DataSets for GSE135314
Status Public on Aug 02, 2022
Title A long non-coding RNA specifically expressed in early embryos programs the metabolic balance in adult mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Long non-coding RNAs (lncRNAs) display higher tissue-specificity than mRNAs. In particular, many lncRNAs are specifically expressed in early embryos, but the physiological functions of most of them remain largely unknown. Here, we show that deficiency of Lncenc1, a lncRNA specifically expressed in early embryos, results in an altered glucose and lipid balance in adult mice. Newly weaned lncenc1-deficient mice have been shown to display higher fasting blood glucose levels and to prefer to use lipids as a fuel source. When mice were fed a normal chow diet (NCD), in which glucose was the main energy source, glucose intolerance and insulin resistance were observed in adult lncenc1-deficient mice. Under high-fat diet (HFD) conditions, however, lncenc1-deficient mice became healthier and could resist food-induced obesity and metabolic disturbances, including liver steatosis and hypercholesterolmia. Furthermore, PPARγ-regulated lipogenesis in liver was reduced in lncenc1-deficient mice fed an HFD, as shown by transcriptome analyses. Interestingly, lncenc1-deficient mouse embryonic fibroblasts (MEFs) showed impaired glycolysis and lipogenesis, suggesting that the metabolic defects may already exist in embryos. Our study provides the first piece of evidence showing the essential roles of embryo-specific lncRNAs in adult metabolism, verifying the “fetal origin” of adult metabolic disorders.
Overall design RNAseq data in wild type and lncenc1 knockout mice livers.
Citation(s) 33059001
Submission date Aug 02, 2019
Last update date Nov 01, 2022
Contact name HONG QIN
Organization name Fudan Univercity
Street address 130# Dongan road
City shanghai
ZIP/Postal code 200032
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (6)
GSM4005156 WT rep-1
GSM4005157 WT rep-2
GSM4005158 WT rep-3
BioProject PRJNA558377
SRA SRP217211

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Supplementary file Size Download File type/resource
GSE135314_fpkm_liver_DEG_3v3.txt.gz 784.9 Kb (ftp)(http) TXT
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