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| Status |
Public on Mar 16, 2020 |
| Title |
RNA-Seq analysis of Tcf7-deficient and -sufficient lung CD8+ TRM cells after influenza infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
T cell factor 1 (Tcf1, encoded by Tcf7) promotes the central memory CD8+ T cell (TCM) differentiation and homeostatic proliferation in lymphoid tissues. It remains unclear if and how Tcf1 regulates the CD103high tissue-resident memory CD8+ T cell (TRM) formation in non-lymphoid tissues. Using an influenza A infection mouse model, we collected Tcf7-deficient and -sufficient lung CD8+ TRM cells for RNA sequencing analysis.
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| Overall design |
To understand the global gene expression profiles in Tcf7-deficient and -sufficient lung CD8+ TRM cells, we infected mice receiving Tcf7-deficient and -sufficient TCR transgenic P14 CD8+ T cells with influenza A virus X31 expressing LCMV epitope GP33-41 (X31-33). Eight days after infection, the mice were injected with PE-conjugated anti-CD8beta to intravascularly label circulating CD8+ T cells. Lung-infiltrating T cells were stained with CD8a and CD103. CD103high Tcf7-deficient and -sufficient P14 CD8+ T cells were FACS-sorted on day 8 after X31 infection for RNA extraction and RNA sequencing (RNA-seq) analysis.
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| Contributor(s) |
Cui G, Wu J |
| Citation(s) |
32268106 |
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| Submission date |
Aug 02, 2019 |
| Last update date |
Jun 15, 2020 |
| Contact name |
Guoliang Cui |
| E-mail(s) |
g.cui@dkfz-heidelberg.de
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| Organization name |
German cancer research center (DKFZ)
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| Street address |
Im Neuenheimer Feld 280
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| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA558369 |
| SRA |
SRP217200 |
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