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Series GSE135289 Query DataSets for GSE135289
Status Public on Aug 03, 2019
Title Generation of Blastocyst-like Structures from Extended Pluripotent Stem Cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary A single mouse blastomere from until 8-cell embryo can generate an entire blastocyst. Whether blastomere-like extended pluripotent stem cells (EPS cells) retain a similar generative capacity remains unknown. Here, we established a 3D differentiation system that enabled the generation of blastocyst-like structures from EPS cells (EPS-blastoids) through lineage segregation and self-organization. EPS-blastoids resembled blastocysts in morphology and cell lineage allocation. EPS-blastoids formation recapitulated key morphogenetic events during preimplantation and early postimplantation development in vitro. Upon transfer, some EPS-blastoids underwent implantation, induced decidualization, and generated live, albeit disorganized, tissues in utero. Single cell and bulk RNA-sequencing analysis revealed that EPS-blastoids contained all three blastocyst cell lineages and shared transcriptional similarity with natural blastocysts. We also provide proof-of-concept that EPS-blastoids can be generated from somatic cells via cellular reprograming. EPS-blastoids provide a unique platform for studying early embryogenesis, and pave the way to generate viable synthetic embryos using cultured cells.
Overall design To exaimine the similarity and difference between EPS-blasotids and nature blastocysts (control)
Contributor(s) Li R, Yu Y, Carlos J, Belmonte I
Citation(s) 31626770
Submission date Aug 02, 2019
Last update date Jan 16, 2020
Contact name gene E
Organization name egene
Street address Julong Street
City Shenzhen
ZIP/Postal code 518118
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (8)
GSM4002774 EPS-blastoid1 [A1]
GSM4002775 EPS-blastoid2 [A2]
GSM4002776 EPS-blastoid3 [A3]
BioProject PRJNA558254
SRA SRP217126

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE135289_RAW.tar 26.0 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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