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| Status |
Public on Aug 03, 2019 |
| Title |
Generation of Blastocyst-like Structures from Extended Pluripotent Stem Cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
A single mouse blastomere from until 8-cell embryo can generate an entire blastocyst. Whether blastomere-like extended pluripotent stem cells (EPS cells) retain a similar generative capacity remains unknown. Here, we established a 3D differentiation system that enabled the generation of blastocyst-like structures from EPS cells (EPS-blastoids) through lineage segregation and self-organization. EPS-blastoids resembled blastocysts in morphology and cell lineage allocation. EPS-blastoids formation recapitulated key morphogenetic events during preimplantation and early postimplantation development in vitro. Upon transfer, some EPS-blastoids underwent implantation, induced decidualization, and generated live, albeit disorganized, tissues in utero. Single cell and bulk RNA-sequencing analysis revealed that EPS-blastoids contained all three blastocyst cell lineages and shared transcriptional similarity with natural blastocysts. We also provide proof-of-concept that EPS-blastoids can be generated from somatic cells via cellular reprograming. EPS-blastoids provide a unique platform for studying early embryogenesis, and pave the way to generate viable synthetic embryos using cultured cells.
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| Overall design |
To exaimine the similarity and difference between EPS-blasotids and nature blastocysts (control)
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| Contributor(s) |
Li R, Yu Y, Carlos J, Belmonte I |
| Citation(s) |
31626770 |
| |
| Submission date |
Aug 02, 2019 |
| Last update date |
Jan 16, 2020 |
| Contact name |
gene E |
| E-mail(s) |
GEO@e-gene.cn
|
| Organization name |
egene
|
| Street address |
Julong Street
|
| City |
Shenzhen |
| ZIP/Postal code |
518118 |
| Country |
China |
| |
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| Platforms (1) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA558254 |
| SRA |
SRP217126 |
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