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| Status |
Public on Jun 01, 2020 |
| Title |
Human macrophage responses to viral-like particles from non-IBD or IBD colonic resections |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Peripheral blood-derived macrophages were stimulated with viral-like particles isolated from colonic resections from patients with Crohn's disease (CD), ulcerative colitis (UC), or non-IBD controls diagnoses. RNAseq was performed to unbiasedly assess the transcriptional responses to these stimuli and revealed highly divergent macrophage transcriptional programs in response to non-IBD compared to IBD VLP.
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| Overall design |
Peripheral blood mononuclear cells from the blood of three healthy human donors were pooled and differentiated into macrophages with M-CSF over the course of seven days. Mature macrophages were treated with viral-like particle (VLP) isolates from colon resections of patients with Crohn's disease (5 individual patient samples), ulcerative colitis (5 individual patient samples), non-IBD diagnoses (5 individual patient samples), or mock treated (3 replicates of mock treatment). RNA was isolated and RNA-seq was carried out for transcriptional analysis.
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| Contributor(s) |
Adiliaghdam F, Digumarthi S, Wong LP, Sadreyev R, Jeffrey KL |
| Citation(s) |
35394816 |
| |
| Submission date |
Aug 01, 2019 |
| Last update date |
Aug 31, 2022 |
| Contact name |
Kate L Jeffrey |
| Organization name |
Massachusetts General Hospital
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| Department |
Medicine
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| Street address |
50 Blossom Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA557840 |
| SRA |
SRP217042 |
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