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Series GSE135223 Query DataSets for GSE135223
Status Public on Jun 01, 2020
Title Human macrophage responses to viral-like particles from non-IBD or IBD colonic resections
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Peripheral blood-derived macrophages were stimulated with viral-like particles isolated from colonic resections from patients with Crohn's disease (CD), ulcerative colitis (UC), or non-IBD controls diagnoses. RNAseq was performed to unbiasedly assess the transcriptional responses to these stimuli and revealed highly divergent macrophage transcriptional programs in response to non-IBD compared to IBD VLP.
Overall design Peripheral blood mononuclear cells from the blood of three healthy human donors were pooled and differentiated into macrophages with M-CSF over the course of seven days. Mature macrophages were treated with viral-like particle (VLP) isolates from colon resections of patients with Crohn's disease (5 individual patient samples), ulcerative colitis (5 individual patient samples), non-IBD diagnoses (5 individual patient samples), or mock treated (3 replicates of mock treatment). RNA was isolated and RNA-seq was carried out for transcriptional analysis.
Contributor(s) Adiliaghdam F, Digumarthi S, Wong LP, Sadreyev R, Jeffrey KL
Citation(s) 35394816
Submission date Aug 01, 2019
Last update date Aug 31, 2022
Contact name Kate L Jeffrey
Organization name Massachusetts General Hospital
Department Medicine
Street address 50 Blossom Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (18)
GSM3995429 Crohn's Disease rep 1
GSM3995430 Crohn's Disease rep 2
GSM3995431 Crohn's Disease rep 3
BioProject PRJNA557840
SRA SRP217042

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE135223_RAW.tar 3.8 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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