|
| Status |
Public on Jan 19, 2020 |
| Title |
Adipocyte serine uptake curbs ROS generation and visceral adiposity [Human] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Obesity, and visceral adiposity in particular, increases the risk of common metabolic diseases, including type 2 diabetes, cardiovascular disease, and several forms of cancer. However, the molecular mechanisms responsible for regional fat storage remain poorly characterized, preventing therapeutic innovation. We here applied a systematic genome-wide screen and translational approach, where human primary preadipocytes were isolated from liposuction aspirate and differentiated. At day 7 of differentiation, cells were treated with BMS-466442 or vehicle (DMSO) for 24 hours. After incubation, cells were lysed, and RNA was purified, DNase treated, and the RNA integrity number was checked. Subsequently, cDNA libraries were generated via the TruSeq Stranded mRNA Library Prep kit and sequenced by Illumina Hiseq 4000.
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| |
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| Overall design |
Total RNA-Seq of Human adipose tissue were sequenced on the Illumina HiSeq4000 platform
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| |
|
| Contributor(s) |
Dankel S, Asen R, Dyer L, Priyanka D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 31, 2019 |
| Last update date |
Feb 05, 2020 |
| Contact name |
Laurence William-Joseph Dyer |
| E-mail(s) |
ldyer2006@gmail.com
|
| Phone |
+420722933092
|
| Organization name |
University of Bergen
|
| Department |
Clinical Institute
|
| Lab |
Hormone Laboratory
|
| Street address |
Haukelandsveien 22
|
| City |
Bergen |
| ZIP/Postal code |
5009 |
| Country |
Norway |
| |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE135156 |
Adipocyte serine uptake curbs ROS generation and visceral adiposity |
|
| Relations |
| BioProject |
PRJNA557598 |
| SRA |
SRP216889 |
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