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| Status |
Public on Aug 01, 2019 |
| Title |
Expression data from D. melanogaster guts with suppression of Imd in progenitors or enterocytes |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used RNA-seq to examine transcriptional proflies of midguts with suppression of Imd in progenitors (e_* samples) or enterocytes (M_* samples) .
We found significant differences between the contributions of enterocyte IMD and progenitor IMD to intestinal homeostasis.
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| Overall design |
Genotype control flies and flies with Imd inhibition were raised on regular food (Bloomington standard food) for 10 days at 29C after eclosion. Guts were dissected for RNA extraction and total RNA, in triplicate, sent to Novogene for library construction and sequencing.
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| Contributor(s) |
Shin M, Foley E |
| Citation missing |
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| |
| Submission date |
Jul 31, 2019 |
| Last update date |
Mar 28, 2021 |
| Contact name |
Edan Foley |
| E-mail(s) |
efoley@ualberta.ca
|
| Organization name |
University of Alberta
|
| Department |
Medical Microbiology and Immunology
|
| Lab |
659 HMRC
|
| Street address |
116 st and 85 ave
|
| City |
Edmonton |
| State/province |
Alberta |
| ZIP/Postal code |
T6G2S2 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
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| Samples (12)
|
| GSM3990080 |
esg[ts]_ImdD30A gut, biological rep1 [e_D_1] |
| GSM3990081 |
esg[ts]_ImdD30A gut, biological rep2 [e_D_2] |
| GSM3990082 |
esg[ts]_ImdD30A gut, biological rep3 [e_D_3] |
| GSM3990083 |
Myo1A[ts] gut, biological rep1 [M_1] |
| GSM3990084 |
Myo1A[ts] gut, biological rep2 [M_2] |
| GSM3990085 |
Myo1A[ts] gut, biological rep3 [M_3] |
| GSM3990086 |
Myo1A[ts]_ImdD30A gut, biological rep1 [M_D_1] |
| GSM3990087 |
Myo1A[ts]_ImdD30A gut, biological rep2 [M_D_2] |
| GSM3990088 |
Myo1A[ts]_ImdD30A gut, biological rep3 [M_D_3] |
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| Relations |
| BioProject |
PRJNA557599 |
| SRA |
SRP216890 |
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