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| Status |
Public on Jan 07, 2020 |
| Title |
RNA-Seq from CA1 pyramidal neurons |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
RNA-Seq from CA1 pyramidal neurons purified by FACS from 4-week-old mice.
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| Overall design |
CA1 pyramidal neurons were labeled with TdTomato by crossing Camk2a-Cre (B6.Cg-Tg(Camk2a-cre)T29-1Stl/J) and Ai14 (B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J mice). The Ai14 reporter line expresses robust tdTomato fluorescence following Cre-mediated recombination. Hippocampi were dissociated from postnatal day 28-32 male Camk2a-Cre +/-, Ai14 +/- mice and TdTomato positive cells isolated by FACS. 3 biological replicates were performed each with cells from 2-4 mice. RNA was extracted from the purified CA1 pyramidal neurons and subjected to RNA-Seq.
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| Contributor(s) |
Sawicka K, Darnell JC, Darnell RB |
| Citation(s) |
31860442 |
| |
| Submission date |
Jul 30, 2019 |
| Last update date |
Jan 07, 2020 |
| Contact name |
Kirsty Sawicka |
| E-mail(s) |
Kirsty.sawicka@cruk.cam.ac.uk
|
| Organization name |
CRUK Cambridge Insititute
|
| Department |
University of Cambridge
|
| Street address |
Robinson Way
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0RE |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE127847 |
The Fragile X protein, FMRP, has a cell-type specific role in CA1 hippocampal neurons to regulate transcripts encoding autism-spectrum proteins and circadian memory |
|
| Relations |
| BioProject |
PRJNA557516 |
| SRA |
SRP216874 |
